Impact of gamma irradiation pretreatment on biochemical and molecular responses of potato growing under salt stress

Abstract Background Previous literatures revealed that gamma rays have an increasing effect on salt tolerance in different plants. In vitro experiment was conducted to study the of (20 Gray) four potato cultivars (Lady Rosetta, Diamante, Gold, and Santana). Results Gamma-treated Santana plantlets were more tolerant salinity as compared other cultivars. It showed a significant increment fresh weight (250% over untreated). Lady Gold higher activity peroxidase (POD) polyphenol oxidase (PPO). Isoenzymes analysis absence POD 3, 4, 5 plantlets. The dye most PODs PPOs bands denser (more active) gamma-treated Both untreated PPO1 Rosetta PPO Genetic marker using ISSR with six primers obvious unique negative positive base pairs mutant control, according primer sequence genotype. 14A efficient genetic between mutated unmutated genotypes. had fingerprint 1430-pb site, which can be selectable for cultivar. An distance cultivar others proved mutation induced because rays. Conclusion We assume irradiation callus by 20-Gy is effective process inducing resistance. However, this finding should verified under field conditions. Graphic